Used for the determination of serum Triglyceride on the Beckman Coulter UniCel DxC600 Synchron Clinical System.

Instrument: Beckman Coulter UniCel DxC600

Specimen Type: Serum

Volume per test: 3uL (min volume 125uL)


TG concentration is measured by a timed endpoint method. The Synchron® System automatically proportions the appropriate sample and reagent volumes into the cuvette at a ratio of 1:100. Triglycerides in the sample are hydrolyzed to glycerol and free fatty acids by the action of lipase. A sequence of three coupled enzymatic steps using glycerol kinase, glycerophosphate oxidase and horseradish peroxidase cause the oxidative coupling of 3,5-dichloro2-hydroxybenzenesulfonic acid with 4-aminoantipyrine to form a red quinoneimine dye. The change in absorbance at 520 nm is directly proportional TG concentration in the sample, and is used by the system to calculate and report TG concentration.

Testing Range: 0.1-11.3 mmol/L