TreFoil Factor 3 (TFF3)
Used for the determination of Trefoil Factor 3 concentration in human citrate plasma using BioVendor Human Trefoil Factor 3 ELISA kit.
Instrument: Manual assay
Specimen Type: Citrate Plasma
Volume per test: 2 x 50uL (min volume 200uL)
This assay employs the sandwich Enzyme-Linked Immunoassay technique. Standards, QC and samples are incubated in microplate wells pre-coated with polyclonal anti-human TFF3 antibody. After incubation and washing, biotin labelled polyclonal anti-human TFF3 antibody is added and incubated. After washing, streptavidin-HRP conjugate is added and incubated. The plate is washed to removed any unbound TFF3 and then the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm with reference wavelength at 630nm. The absorbance is proportional to the concentration of TFF3.
Testing Range: 0.15-2.4 ng/mL