Used for the quantitative determination of sFlt-1 in human serum via electrochemiluminescent immunoassay on the Roche Cobas automated analyzer.
Instrument: Roche Cobas
Specimen Type: Serum
Volume per test: 20µL (min volume 150µL)
The total duration of this sandwich principle assay is 18 minutes.
First incubation: 20µL of sample, a biotinylated monoclonal sFlt-1-specific antibody, and a monoclonal sFlt-1-specific antibody labeled with a ruthenium complex react to form a sandwich complex.
Second incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin.
The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultoplier.
Results are determined via a calibration curve which is instrument-specifically generated by 2-point calibration and a master curve provided via the reagent barcode.
Testing Range: 10-85000 pg/mL