Salivary C-Reactive Protein
Used for the determination of C-Reactive Protein (CRP) concentration in human saliva using the Salimetircs Salivary C-Reactive Protein ELISA kit (1-3302).
Instrument: Manual assay
Specimen Type: Saliva
Volume per test: 15μL (min volume 100μL)
This assay employs the indirect sandwich ELISA technique. A sandwich is formed when the pre-coated capture anti-CRP antibody present on the plate binds CRP in standards and samples, which is then bound by the anti-CRP detection antibody linked to horseradish peroxidase. After incubation, unbound components are washed away. Bound CRP Antibody Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue colour. A yellow colour is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm.
Testing Range: 4.6-130.43 pmol/L