Used for the quantitative determination of ProBNP II in human serum and lithium-heparin plasma via electrochemiluminescent immunoassay on the Roche Cobas automated analyzer.
Instrument: Roche Cobas
Specimen Type: Serum; Lithium-Heparin Plasma
Volume per test: 15uL (min volume 125uL)
The total duration of this sandwich principle assay is 18 minutes.
First incubation: Antigen in 15uL of sample, a biotinylated NT-proBNP-specific antibody, and an NT-proBNP-specific antibody labeled with a ruthenium complex react to form a sandwich complex.
Second incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin.
The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed with ProCell M. Application of a voltage to the electrode induces chemiluminescent emission which is measured by a photo multiplier.
Results are determined via a calibration curve which is instrument-specifically generated by 2-point calibration and a master curve provided via the reagent barcode.
Testing Range: 0.6-4130 pmol/L