Used for the determination of Osteoprotegerin (OPG) concentration in human citrate plasma using BioVendor Human Osteoprotegerin ELISA.
Instrument: Manual assay
Specimen Type: Citrate Plasma
NOTE: Unable to report results for hemolyzed or lipemic specimens.
Volume per test: 2 x 100uL (min volume 300uL)
This assay employs the sandwich enzyme immunoassay technique. Standards, QC and samples are incubated in microplate wells pre-coated with the polyclonal anti-human OPG antibody. After incubation and washing, biotin labelled polyclonal anti-human OPG antibody is added and incubated. After washing, streptavidin-HRP conjugate is added and incubated. The plate is washed to remove any unbound OPG and then the remaining conjugate is allowed to react with the substrate solution. The reaction is stopped by addition of acidic solution and absorption of the resulting yellow product is measured at 450nm with a reference wavelength set to 630nm. The absorbance is proportional to the concentration of OPG.
Testing Range: 3.0-60.0 pmol/L