Used for the quantitative determination of IL-6 in human serum via electrochemiluminescent immunoassay on the Roche Cobas analytics system.
Instrument: Roche Cobas
Specimen Type: Serum
Volume per test: 30µL (min volume 150µL)
The total duration of this sandwich principle assay is 18 minutes.
First incubation: 30µL of sample are incubated with a biotinylated monoclonal IL-6 antibody.
Second incubation: After addition of a monoclonal IL-6-specific antibody labeled with ruthenium complex and streptavidin-coated microparticles, the antibodies form a sandwich complex with the antigen of the sample.
The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier.
Results are determined via a calibration curve which is instrument-specifically generated by 2-point calibration and a master curve provided via the reagent barcode.
Testing Range: 1.5-5000 pg/mL