Insulin-Growth Factor Binding Protein 2 (IGFBP-2)
Used for the determination of Insulin-Growth Factor Binding Protein 2 concentration in human EDTA plasma using R&D Systems Human IGFBP-2 Quantikine ELISA kit.
Instrument: Manual assay
Specimen Type: EDTA Plasma
Volume per test: 2 x 10uL (min volume 50uL)
This assay employs the solid-phase sandwich ELISA technique. A monoclonal antibody specific for IGFBP-2 is pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGFBP-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IGFBP-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IGFBP-2 bound in the initial step. Colour development is stopped and the intensity of the colour is measured.
Testing Range: 0.313-20 ng/mL