Used for the determination of serum HDL Cholesterol on the Beckman Coulter UniCel DxC600 Synchron Clinical System.
Instrument: Beckman Coulter UniCel DxC600
Specimen Type: Serum
NOTE: Extremely lipemic samples may give falsely low results.
Volume per test: 3uL (min volume 125uL)
This test is a two reagent homogenous system for selective measurement of serum or plasma HDL-Cholesterol in the presence of other lipoprotein particles. In phase one of the assay, free cholesterol in non-HDL-lipoproteins is solubilized and consumed by cholesterol oxidase, peroxidase, and DSBmT to generate a colorless end product. In phase two of the assay, a unique detergent selectively solubilizes HDL- lipoproteins to release HDL cholesterol which reacts with cholesterol esterase, cholesterol oxidase and a chromogen system to yield a blue color complex which can be measured bichromatically at 600/700nm. The resulting increase in absorbance is directly proportional to the HDL-C concentration in the sample.
Testing Range: 0.13-3.49 mmol/L