Growth Differentiation Factor 15 (GDF-15)
Used for the determination of GDF-15 concentration in human serum using R&D Quantikine ELISA (DG150).
Instrument: Manual assay
Specimen Type: Serum
Volume per test: 2 x 50uL (min volume 200uL)
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human GDF-15 is precoated onto the microplate. Standards and samples are pipetted into the wells and any GDF-15 present is bound by the immobilized antibody. After washing away unbound substances, an enzyme-linked polyclonal antibody specific for human GDF-15 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of GDF-15 bound in the initial step. Colour development is stopped and the intensity of the colour is measured.
Testing Range: 23.4-1500 pg/mL