Glucose Roche Cobas
Used for the quantitative determination of glucose in human serum via enzymatic in vitro assay on the Cobas automated analyzer.
Instrument: Roche Cobas
Specimen Type: Serum
Volume per test: 2uL (min volume 125uL)
This UV test involves two additive steps: Specimen and R1, then addition of R2 which initiates the reaction:
Glucose + ATP → G-6-P + ADP
Glucose-6-phosphate dehydrogenase oxidizes glucose-6-phosphate in the presence of NADP to gluconate-6-phosphate. No other carbohydrate is oxidized. The rate of NADPH formation during the reaction is directly proportional to the glucose concentration and can be measured photometrically.
Testing Range: 0.1-41.6 mmol/L