Brain Derived Neurotrophic Factor (BDNF)

Brain Derived Neurotrophic Factor (BDNF)

Used for the determination of Brain Derived Neurotrophic Factor (BDNF) concentration in human serum using R&D Systems QuantikineĀ® Human BDNF ELISA kit.

Instrument: Manual assay

Specimen Type: Serum

Volume per test: 2 x 50uL (min volume 200uL)

Principle:

This assay uses a quantitative sandwich enzyme immunoassay technique. Monoclonal antibody specific for BDNF is pre-coated onto the microplate. Standards and samples are pipetted into the wells and BDNF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for BDNF is added. After washing to remove unbound antibody-enzyme reagent, substrate solution is added and colour development occurs in proportion to the amount of BDNF bound in the initial step. Colour development is stopped and colour intensity is measured.

Testing Range: 62.5-4000 pg/mL