Used for the determination of Angiopoietin-2 concentration in human serum using R&D Systems Quantikine Human Angiopoietin-2 ELISA kit.
Instrument: Manual assay
Specimen Type: Serum
Volume per test: 2 x 50uL (min volume 200uL)
This assay employs the solid-phase sandwich ELISA technique. A monoclonal antibody specific for Ang-2 is pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ang-2 present is bound by the immobilized antibody. After washing away unbound substances, an enzyme-linked monoclonal antibody specific for Ang-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of Ang-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured.
Testing Range: 46.9-3000 pg/mL