STAT High sensitive Troponin I (hsTNI)

STAT High sensitive Troponin I (hsTNI)

Used for the quantitative determination of Stat High Sensitive Troponin I in human serum and plasma via chemiluminescent microparticle immunoassay on the Architect i1000SR automated analyzer.

Instrument: Architect i1000SR

Specimen Type: Serum; EDTA Plasma; Lithium-Heparin Plasma

Volume per test: 210uL (min volume 250uL)

Principle:

The Architect STAT High Sensitive Troponin-I assay is a 2-step immunoassay to determine the presence of cardiac Troponin I (cTNI) in human plasma and serum using chemiluminescent microparticle immunoassay technology.

In the first step, sample and anti-troponin I antibody-coated paramagnetic microparticles are combined. Cardiac troponin I present in the sample binds to the anti-troponin I coated microparticles.

After incubation and wash, anti-troponin I acridinium-labeled conjugate is added in the second step. Following another incubation and wash, pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).

Testing Range: 1-50000 ng/L

Limitations: Serum specimens from individuals on anticoagulant therapy may show inconsistent results due to incomplete clotting.